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New oligopeptide immunoglobulin G test for human parvovirus B19 antibodies

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Schwarz, T. F. ; Modrow, Susanne ; Hottenträger, B. ; Höflacher, B. ; Jäger, G. ; Scharti, W. ; Sumazaki, R. ; Wolf, Hans J. ; Middeldorp, J. ; Roggendorf, M.
Date of publication of this fulltext: 12 Apr 2011 08:02


A new, highly sensitive and specific enzyme immunoassay using oligopeptides as antigen (enzyme-linked immunosorbent assay [ELISA] B19-OP) for detecting parvovirus B19-specific immunoglobulin G (IgG) was established. As antigens, B19-specific oligopeptides of 24 and 30 kDa derived from a 196-kDa fusion protein of beta-galactosidase and viral capsid protein (VPI) of B19 after CNBr cleavage and ...


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