Jilg, W., Voltz, R., Markert-Hahn, C., Mairhofer, H., Münz, I. und Wolf, Hans J.
(1991)
Expression of class I major histocompatibility complex antigens in Epstein-Barr virus-carrying lymphoblastoid cell lines and Burkitt lymphoma cells.
Cancer research 51 (1), S. 27-32.
Veröffentlichungsdatum dieses Volltextes: 13 Apr 2011 09:09Zum PubMed-Eintrag dieses Artikels
Zusammenfassung
Epstein-Barr virus (EBV) carrying lymphoblastoid cell lines (LCLs) and EBV-positive Burkitt lymphoma (BL) cells were compared for their expression of class I antigens of the major histocompatibility complex. Five common BL lines, LCLs, pokeweed mitogen-stimulated blasts and resting B-cells from healthy donors, and eight pairs of BL cells and LCLs, each pair originating from one patient, were ...
Zusammenfassung
Epstein-Barr virus (EBV) carrying lymphoblastoid cell lines (LCLs) and EBV-positive Burkitt lymphoma (BL) cells were compared for their expression of class I antigens of the major histocompatibility complex. Five common BL lines, LCLs, pokeweed mitogen-stimulated blasts and resting B-cells from healthy donors, and eight pairs of BL cells and LCLs, each pair originating from one patient, were tested. Quantitative analysis was performed using a radioimmunoassay; qualitative aspects were studied by one- and two-dimensional gel electrophoresis. In general, LCLs expressed significantly higher amounts of class I antigens than BL cells, the latter showing class I densities similar to or lower than peripheral resting B-cells. From analysis of the expression of class I-specific RNA, there is some evidence that class I antigen expression is regulated on the transcriptional level. In two BL cells studied, class I expression could be enhanced by gamma-interferon, whereas the corresponding LCLs seemed to be refractory to this treatment. One- and two-dimensional gel electrophoresis showed that in some BL lines, in addition to the generally lower class I expression, distinct class I specificities were down-regulated. None of these alterations in class I expression was EBV specific; however, they may well play a role in the recognition of BL cells and LCLs by cellular immune mechanisms. Thus, down-regulation of class I antigens may contribute to the resistance of BL cells to cytotoxic T-lymphocytes, whereas their enhanced expression may improve the recognition of EBV-infected LCLs.
Bibliographische Daten exportieren
| Dokumentenart: | Artikel |
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| Datum: | 1991 |
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| Institutionen: | Medizin > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
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| Identifikationsnummer: | |
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| Klassifikation: | | Notation | Art |
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| Blotting, Northern | MESH | | Burkitt Lymphoma/immunology | MESH | | Cell Line | MESH | | Cell Transformation, Viral | MESH | | Electrophoresis, Gel, Two-Dimensional | MESH | | Histocompatibility Antigens Class I/metabolism | MESH | | Humans | MESH | | Interferon-gamma/pharmacology | MESH | | Lymphocytes/microbiology | MESH | | Major Histocompatibility Complex | MESH | | Molecular Weight | MESH | | Pokeweed Mitogens/pharmacology | MESH | | Precipitin Tests | MESH | | RNA, Messenger/genetics | MESH | | Tumor Virus Infections/immunology | MESH |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Unbekannt / Keine Angabe |
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| An der Universität Regensburg entstanden: | Unbekannt / Keine Angabe |
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| Dokumenten-ID: | 20469 |
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