Expression of class I major histocompatibility complex antigens in Epstein-Barr virus-carrying lymphoblastoid cell lines and Burkitt lymphoma cells

URN to cite this document:

urn:nbn:de:bvb:355-epub-204694 Copy

Jilg, W. ; Voltz, R. ; Markert-Hahn, C. ; Mairhofer, H. ; Münz, I. ; Wolf, Hans J.

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Date of publication of this fulltext: 13 Apr 2011 09:09

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Details

Item type:	Article
Journal or Publication Title:	Cancer research
Publisher:	American Association for Cancer Research : AACR
Volume:	51
Number of Issue or Book Chapter:	1
Page Range:	pp. 27-32
Date:	1991
Institutions:	Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Identification Number:	Value Type 1899042 PubMed ID
Classification:	Notation Type Blotting, Northern MESH Burkitt Lymphoma/immunology MESH Cell Line MESH Cell Transformation, Viral MESH Electrophoresis, Gel, Two-Dimensional MESH Histocompatibility Antigens Class I/metabolism MESH Humans MESH Interferon-gamma/pharmacology MESH Lymphocytes/microbiology MESH Major Histocompatibility Complex MESH Molecular Weight MESH Pokeweed Mitogens/pharmacology MESH Precipitin Tests MESH RNA, Messenger/genetics MESH Tumor Virus Infections/immunology MESH
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Unknown
Created at the University of Regensburg:	Unknown
Item ID:	20469

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Abstract

Epstein-Barr virus (EBV) carrying lymphoblastoid cell lines (LCLs) and EBV-positive Burkitt lymphoma (BL) cells were compared for their expression of class I antigens of the major histocompatibility complex. Five common BL lines, LCLs, pokeweed mitogen-stimulated blasts and resting B-cells from healthy donors, and eight pairs of BL cells and LCLs, each pair originating from one patient, were ...

Abstract

Epstein-Barr virus (EBV) carrying lymphoblastoid cell lines (LCLs) and EBV-positive Burkitt lymphoma (BL) cells were compared for their expression of class I antigens of the major histocompatibility complex. Five common BL lines, LCLs, pokeweed mitogen-stimulated blasts and resting B-cells from healthy donors, and eight pairs of BL cells and LCLs, each pair originating from one patient, were tested. Quantitative analysis was performed using a radioimmunoassay; qualitative aspects were studied by one- and two-dimensional gel electrophoresis. In general, LCLs expressed significantly higher amounts of class I antigens than BL cells, the latter showing class I densities similar to or lower than peripheral resting B-cells. From analysis of the expression of class I-specific RNA, there is some evidence that class I antigen expression is regulated on the transcriptional level. In two BL cells studied, class I expression could be enhanced by gamma-interferon, whereas the corresponding LCLs seemed to be refractory to this treatment. One- and two-dimensional gel electrophoresis showed that in some BL lines, in addition to the generally lower class I expression, distinct class I specificities were down-regulated. None of these alterations in class I expression was EBV specific; however, they may well play a role in the recognition of BL cells and LCLs by cellular immune mechanisms. Thus, down-regulation of class I antigens may contribute to the resistance of BL cells to cytotoxic T-lymphocytes, whereas their enhanced expression may improve the recognition of EBV-infected LCLs.

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