Characterization of a urea optical sensor based on polypyrrole

DOI to cite this document:: 10.5283/epub.20476

de Marcos, Susana ; Hortigüela, Rubén ; Gatbán, Javier ; Castillo, Juan R. ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 18 Apr 2011 13:26

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Abstract

A new optical sensor for urea determination is presented. It is based on the enzymatic reaction with urease, which is first photoimmobilized with polyacrylamide onto a chemically polymerized polypyrrole (PPy) film. The main advantage of this sensor is that no indicator dye or pH indicator is needed, because PPy itself acts as the support and the indicator. These PPy films show an absorbance ...