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Detection of hepatitis B virus in serum using amplification of viral DNA by means of the polymerase chain reaction

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Sumazaki, R. ; Motz, M. ; Wolf, Hans ; Heinig, J. ; Jilg, Wolfgang ; Deinhardt, F.
Date of publication of this fulltext: 13 Apr 2011 08:45


A new assay was developed for the detection of hepatitis B virus (HBV) in human serum using amplification of a short viral DNA sequence by means of the polymerase chain reaction. As little as 0.4 fg viral DNA, corresponding to about 130 genome equivalents, per ml serum could be detected after the amplification procedure. This assay detected viral DNA in a number of patients with proven or ...


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