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Truncated versions of the two major Epstein-Barr viral glycoproteins (gp250/350) are secreted by recombinant Chinese hamster ovary cells

URN to cite this document:
urn:nbn:de:bvb:355-epub-205403
Motz, M. ; Deby, G. ; Wolf, Hans J.
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Date of publication of this fulltext: 15 Apr 2011 08:40


Abstract

The expression of the two Epstein-Barr virus (EBV) major membrane proteins gp250/350 (MA-BLLF1) on the surface of recombinant CHO clones cannot be amplified by methotrexate (MTX) selection, perhaps due to toxic effects of these membrane proteins. After removal of sequences encoding the part of the glycoproteins responsible for membrane anchorage, the gp250/350 is secreted into the medium. ...

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