Truncated versions of the two major Epstein-Barr viral glycoproteins (gp250/350) are secreted by recombinant Chinese hamster ovary cells

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urn:nbn:de:bvb:355-epub-205403 Copy

Motz, M. ; Deby, G. ; Wolf, Hans J.

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Date of publication of this fulltext: 15 Apr 2011 08:40

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Details

Item type:	Article
Date:	1987
Institutions:	Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Identification Number:	Value Type 2826297 PubMed ID
Classification:	Notation Type Animals MESH Antigens, Viral/isolation & purification MESH Clone Cells/secretion MESH Cloning, Molecular MESH Cricetinae MESH Cricetulus MESH Female MESH Gene Amplification MESH Herpesvirus 4, Human/immunology MESH Ovary MESH Plasmids MESH Viral Matrix Proteins/secretion MESH Viral Vaccines/isolation & purification MESH
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Unknown
Created at the University of Regensburg:	Unknown
Item ID:	20540

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Abstract

The expression of the two Epstein-Barr virus (EBV) major membrane proteins gp250/350 (MA-BLLF1) on the surface of recombinant CHO clones cannot be amplified by methotrexate (MTX) selection, perhaps due to toxic effects of these membrane proteins. After removal of sequences encoding the part of the glycoproteins responsible for membrane anchorage, the gp250/350 is secreted into the medium. ...

Abstract

The expression of the two Epstein-Barr virus (EBV) major membrane proteins gp250/350 (MA-BLLF1) on the surface of recombinant CHO clones cannot be amplified by methotrexate (MTX) selection, perhaps due to toxic effects of these membrane proteins. After removal of sequences encoding the part of the glycoproteins responsible for membrane anchorage, the gp250/350 is secreted into the medium. Following selection with MTX, this construct allows the amplification of the expression products. Besides the possible use of these proteins in protection experiments, they can also be used as antigens for diagnosis, which opens an efficient approach for control of EBV-related neoplasias by early therapy.

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Metadata last modified: 03 Jun 2018 04:50

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