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Determination of the activity of catalase using a europium(III)-tetracycline-derived fluorescent substrate

Wu, Meng, Lin, Zhihong and Wolfbeis, Otto S. (2003) Determination of the activity of catalase using a europium(III)-tetracycline-derived fluorescent substrate. Analytical Biochemistry 320 (1), pp. 129-135.

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Date of publication of this fulltext: 13 May 2011 08:15

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Other URL: http://www.sciencedirect.com/science/article/B6W9V-49037CX-2/2/83c4ea16e74eab6921b807d141939d5c


Abstract

A one-step method is described for the fluorometric determination of the activity of the enzyme catalase (EC 1.11.1.6.), based on the finding that H2O2 in the europium (III)-tetracycline-hydrogen peroxide system is consumed by catalase. This is accompanied by a large decrease in both fluorescence intensity and decay time. The limit of detection (LOD; at S/N=3) for catalase at 30 °C for a 10-min ...

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Item type:Article
Date:2003
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:
ValueType
10.1016/S0003-2697(03)00356-7DOI
Keywords:Catalase; Europium; Tetracycline; Hydrogen peroxide
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Item ID:20736
Owner only: item control page

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