Determination of the activity of catalase using a europium(III)-tetracycline-derived fluorescent substrate

DOI to cite this document:: 10.5283/epub.20736

Wu, Meng ; Lin, Zhihong ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 13 May 2011 08:15

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Abstract

A one-step method is described for the fluorometric determination of the activity of the enzyme catalase (EC 1.11.1.6.), based on the finding that H2O2 in the europium (III)-tetracycline-hydrogen peroxide system is consumed by catalase. This is accompanied by a large decrease in both fluorescence intensity and decay time. The limit of detection (LOD; at S/N=3) for catalase at 30 °C for a 10-min ...