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Hyperthermostable acetyl xylan esterase

Drzewiecki, Katharina ; Angelov, Angel ; Ballschmiter, Meike ; Tiefenbach, Klaus-Jürgen ; Sterner, Reinhard ; Liebl, Wolfgang



Abstract

An esterase which is encoded within a Thermotoga maritima chromosomal gene cluster for xylan degradation and utilization was characterized after heterologous expression of the corresponding gene in Escherichia coli and purification of the enzyme. The enzyme, designated AxeA, shares amino acid sequence similarity and its broad substrate specificity with the acetyl xylan esterase from Bacillus ...

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