Set of fluorochromophores in the wavelength range from 450 to 700 nm and suitable for labeling proteins and amino-modified DNA

DOI zum Zitieren dieses Dokuments:: 10.5283/epub.20763

Wetzl, Bianca ; Gruber, Michaela ; Oswald, Bernhard ; Dürkop, Axel ; Weidgans, Bernhard ; Probst, Mario ; Wolfbeis, Otto S.

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Veröffentlichungsdatum dieses Volltextes: 13 Mai 2011 08:18

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Zusammenfassung

We describe the synthesis, purification, and spectral properties of new dyes and reactive labels. They absorb in the visible range between 450 and 700 nm and display analytically useful fluorescence. They were made amino-reactive by esterification with N-hydroxysuccinimide (NHS). The resulting oxysuccinimide (OSI) esters were covalently linked to the amino groups of human serum albumin (HSA) or ...