The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro

URN to cite this document:

urn:nbn:de:bvb:355-epub-209769 Copy

DOI to cite this document:

10.5283/epub.20976 Copy

Lechler, Philipp ; Renkawitz, Tobias ; Campean, Valentina ; Balakrishnan, Sanjeevi ; Tingart, Markus ; Grifka, Joachim ; Schaumburger, Jens

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License: Creative Commons Attribution 3.0 PDF - Published Version (1MB)

Date of publication of this fulltext: 25 May 2011 09:59

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Details

Item type:	Article
Journal or Publication Title:	BMC Cancer
Publisher:	BioMed Central
Volume:	11
Page Range:	p. 120
Date:	2011
Institutions:	Medicine > Lehrstuhl für Orthopädie
Projects:	Open Access Publizieren (DFG)
Identification Number:	Value Type 10.1186/1471-2407-11-120 DOI 21457573 PubMed ID
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Partially
Item ID:	20976

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Abstract

Background Chondrosarcoma is virtually resistant to chemotherapy and radiation therapy. Survivin, the smallest member of the inhibitor of apoptosis protein family, is a critical factor for tumor progression and resistance to conventional therapeutic approaches in a wide range of malignancies. However, the role of survivin in chondrosarcoma has not been well studied. We examined the importance of ...

Abstract

Background
Chondrosarcoma is virtually resistant to chemotherapy and radiation therapy. Survivin, the smallest member of the inhibitor of apoptosis protein family, is a critical factor for tumor progression and resistance to conventional therapeutic approaches in a wide range of malignancies. However, the role of survivin in chondrosarcoma has not been well studied. We examined the importance of survivin gene expression in chondrosarcoma and analysed its influences on proliferation, apoptosis and resistance to chemotherapy in vitro.
Methods
Resected chondrosarcoma specimens from which paraffin-embedded tissues could be extracted were available from 12 patients. In vitro experiments were performed in human chondrosarcoma cell lines SW1353 and Hs819.T. Immunohistochemistry, immunoblot, quantitative PCR, RNA interference, gene-overexpression and analyses of cell proliferation and apoptosis were performed.
Results
Expression of survivin protein was detected in all chondrosarcoma specimens analyzed, while undetectable in adult human cartilage. RNA interference targeting survivin resulted in a G2/M-arrest of the cell cycle and led to increased rates of apoptosis in chondrosarcoma cells in vitro. Overexpression of survivin resulted in pronounced resistance to doxorubicin treatment.
Conclusions
These findings indicate that survivin plays a role in the pathogenesis and pronounced chemoresistance of high grade chondrosarcoma. Survivin antagonizing therapeutic strategies may lead to new treatment options in unresectable and metastasized chondrosarcoma.

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Metadata last modified: 26 Nov 2020 06:43

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