Polarization immunoassays using reactive ruthenium metal-ligand complexes as luminescent labels

DOI to cite this document:: 10.5283/epub.21015

Dürkop, Axel ; Lehmann, Frank ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 06 Jun 2011 11:39

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Abstract

The first competitive fluorescence polarization immunoassay using ruthenium metal-ligand complexes (5-MC and 55-DC) as labels is described. These were newly synthesized and characterized in terms of their spectra, their covalent linkage to proteins, and their use in both homogeneous and competitive immunoassays. Linkage to proteins was achieved by the N-hydroxysuccinimide ester method, which was ...