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Polarization immunoassays using reactive ruthenium metal-ligand complexes as luminescent labels

Dürkop, Axel, Lehmann, Frank and Wolfbeis, Otto S. (2002) Polarization immunoassays using reactive ruthenium metal-ligand complexes as luminescent labels. Analytical and Bioanalytical Chemistry 372 (5-6), pp. 688-694.

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Date of publication of this fulltext: 06 Jun 2011 11:39

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Abstract

The first competitive fluorescence polarization immunoassay using ruthenium metal-ligand complexes (5-MC and 55-DC) as labels is described. These were newly synthesized and characterized in terms of their spectra, their covalent linkage to proteins, and their use in both homogeneous and competitive immunoassays. Linkage to proteins was achieved by the N-hydroxysuccinimide ester method, which was ...

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Item type:Article
Date:2002
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:
ValueType
10.1007/s00216-002-1232-zDOI
Keywords:Ruthenium metal-ligand complex ·Polarization probe ·Fluorescence polarization immunoassay
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Item ID:21015
Owner only: item control page

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