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Synthesis, Spectral Properties, and Detection Limits of Reactive Squaraine Dyes, a New Class of Diode Laser Compatible Fluorescent Protein Labels

Oswald, Bernhard ; Patsenker, Leonid ; Duschl, Josef ; Szmacinski, Henryk ; Wolfbeis, Otto S. ; Terpetschnig, Ewald
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Date of publication of this fulltext: 06 Jun 2011 13:03


Abstract

We describe the synthesis and spectral characterization of two reactive long-wavelength fluorescence labels (Sq635-m and Sq635-b), having either one or two N-hydroxysuccinimidyl esters. Both are squaraine derivatives and consist of a cyanine-type chromophore and a central squarate bridge. To improve water solubility, we introduced two sulfonic acid groups into the heterocyclic ring systems, and ...

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