Application of non-specific fluorescent dyes for monitoring enantio-selective ligand binding to molecularly imprinted polymers

DOI to cite this document:: 10.5283/epub.21068

Piletsky, Sergey A. ; Terpetschnig, Ewald ; Andersson, Håkan S. ; Nicholls, Ian A. ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 06 Jun 2011 13:19

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Abstract

The displacement of non-specific dyes from molecularly imprinted polymer (MIP) chromatographic stationary phases has been used for the detection and quantification of ligand-polymer binding events. A blank polymer and an L-phenylalaninamide-imprinted polymer were prepared using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as a crosslinker. The MIP is first loaded ...