Capacitive monitoring of protein immobilization and antigen–antibody reactions on monomolecular alkylthiol films on gold electrodes

DOI to cite this document:: 10.5283/epub.21088

Mirsky, Vladimir M. ; Riepl, Michael ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 06 Jun 2011 13:48

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Item type:	Article
Journal or Publication Title:	Biosensors and Bioelectronics
Publisher:	Elsevier
Volume:	12
Number of Issue or Book Chapter:	9-10
Page Range:	pp. 977-989
Date:	1997
Institutions:	Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Keywords:	protein immobilization, self-assembly, immunosensor, impedance, capacitive sensors
Dewey Decimal Classification:	500 Science > 540 Chemistry & allied sciences
Status:	Published
Refereed:	Unknown
Created at the University of Regensburg:	Unknown
Item ID:	21088

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Abstract

Self-assembled monolayers of v-mercaptohexadecanoic acid and vmercaptohexadecylamine on gold electrodes are stable at neutral pH and display pure capacitive behavior at frequencies around 20 Hz. Different methods of covalent immobilization of proteins on these monolayers are compared. Various reagents including succinimides, thionylchloride, p-nitrophenol and carbodiimides were used to activate ...

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