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Capacitive monitoring of protein immobilization and antigen–antibody reactions on monomolecular alkylthiol films on gold electrodes

Mirsky, Vladimir M. ; Riepl, Michael ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 06 Jun 2011 13:48


Abstract

Self-assembled monolayers of v-mercaptohexadecanoic acid and vmercaptohexadecylamine on gold electrodes are stable at neutral pH and display pure capacitive behavior at frequencies around 20 Hz. Different methods of covalent immobilization of proteins on these monolayers are compared. Various reagents including succinimides, thionylchloride, p-nitrophenol and carbodiimides were used to activate ...

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