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Regulation of TNF-alpha, IL-1 and IL-6 synthesis in differentiating human monoblastoid leukemic U937 cells

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Hass, R. ; Lonnemann, G. ; Männel, Daniela N. ; Topley, N. ; Hartmann, A. ; Köhler, L. ; Resch, K. ; Goppelt-Strübe, M.
Date of publication of this fulltext: 20 Jun 2011 06:18


The human monoblastoid tumor cell line U937 was induced to differentiate along the monocyte/macrophage lineage by treatment with 5 x 10(-9) M 12-O-tetradecanoyl phorbol-13-acetate (TPA). Between 2 h and 4 h following TPA-treatment U937 cells started to release significant amounts of TNF-alpha which remained detectable until 8-10 days. A significant IL-1 beta release was measured 24 h-48 h post ...


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