Hensel, G., Männel, Daniela N., Pfizenmaier, K. and Krönke, M.
(1987)
Autocrine stimulation of TNF-alpha mRNA expression in HL-60 cells.
Lymphokine research 6 (2), pp. 119-125.
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Abstract
We have employed a human promyelocytic leukemic cell line, HL-60, to investigate the conditions that regulate TNF-alpha gene expression in vitro. Using a cloned TNF-alpha specific cDNA probe, we show by Northern blot analysis that TNF-alpha mRNA rapidly accumulates in HL-60 cells following treatment with phorbol myristate acetate (PMA). TNF-alpha mRNA levels peak at 1-2 hours and then decline to ...
Abstract
We have employed a human promyelocytic leukemic cell line, HL-60, to investigate the conditions that regulate TNF-alpha gene expression in vitro. Using a cloned TNF-alpha specific cDNA probe, we show by Northern blot analysis that TNF-alpha mRNA rapidly accumulates in HL-60 cells following treatment with phorbol myristate acetate (PMA). TNF-alpha mRNA levels peak at 1-2 hours and then decline to base-line levels within 8 hours. TNF-alpha protein levels as detected in the supernatants of PMA-stimulated HL-60 cells peak at 2 hours and decline within 24 hours. Cytokines of recombinant source like interferon-alpha and -gamma, and, more intruigingly, TNF-beta as well as TNF-alpha itself are also able to induce transient TNF-alpha mRNA expression in HL-60 cells. In the presence of a protein kinase inhibitor, neither PMA nor any of the cytokines used are able to induce TNF-alpha mRNA accumulation, indicating that protein kinases may be crucially involved in signal transduction leading to activation of the TNF-alpha gene. The data presented provide new insights into the control of TNF-alpha gene expression suggesting regulatory functions of T- and B-cell derived lymphokines as well as a TNF-alpha-mediated positive feedback mechanism.
Export bibliographical data
| Item type: | Article |
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| Date: | 1987 |
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| Institutions: | Medicine > Lehrstuhl für Immunologie |
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| Identification Number: | | Value | Type |
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| 3108589 | PubMed ID |
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| Classification: | | Notation | Type |
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| Cell Line | MESH | | Gene Expression Regulation/drug effects | MESH | | Glycoproteins/pharmacology | MESH | | Humans | MESH | | Interferon Type I/pharmacology | MESH | | Interferon-gamma/pharmacology | MESH | | Macrophage Activation | MESH | | RNA, Messenger/metabolism | MESH | | Tetradecanoylphorbol Acetate/pharmacology | MESH | | Tumor Necrosis Factor-alpha | MESH |
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| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine |
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| Status: | Published |
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| Refereed: | Yes, this version has been refereed |
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| Created at the University of Regensburg: | Unknown |
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| Item ID: | 21189 |
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