Antigen-specific electrophoretic cell separation (ASECS): isolation by human T and B lymphocyte subpopulations by free-flow electrophoresis after reaction with antibodies

URN to cite this document:: urn:nbn:de:bvb:355-epub-219894
DOI to cite this document:: 10.5283/epub.21989

Hansen, Ernil ; Hannig, Kurt

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Date of publication of this fulltext: 05 Sep 2011 13:33

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Abstract

The electrophoretic mobility of human lymphocytes can be reduced by incubation with surface antigen specific antibodies under non-capping conditions. This renders subpopulations of human peripheral blood lymphocytes accessible to separation by free-flow electrophoresis. After reaction of lymphocyte preparations with anti-IgM antibody and a fluorescent second antibody, B lymphocytes showed a ...