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- URN zum Zitieren dieses Dokuments:
- urn:nbn:de:bvb:355-epub-220585
- DOI zum Zitieren dieses Dokuments:
- 10.5283/epub.22058
Zusammenfassung
Quantitation of Ca+ and H+ activities within cells using presently available fluorescent probes is optimal when the fluorescence signal is calibrated in situ after each experiment. Fura-2 and 2',7'-bis(2-carboxy-ethyl)-5,6-carboxyfluoroscein (BCECF) are difficult to calibrate in freshly dissociated adult cardiac myocytes because calibration procedures produce cellular hypercontracture. In situ ...
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