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Thermal protein unfolding in photo-activated adenylate cyclase nano-clusters from the amoeboflagelate Naegleria gruberi NEG-M strain

Penzkofer, Alfons ; Stierl, Manuela ; Hegemann, Peter ; Kateriya, Suneel


The photo-activated adenylate cyclase (nPAC) protein from the amoeboflagellate Naegleria gruberi NEG-M strain consists of a BLUF domain (sensor of blue light using flavin) and a cyclase homology domain (CHD). The nPAC thermal stability is determined by its proteinunfolding behavior which is quantified by the protein melting temperature and protein melting time. The proteinunfolding in nPAC ...


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