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Fluorescence-based bacterial overlay method for simultaneous in situ quantification of surface-attached bacteria

Müller, Rainer ; Gröger, G. ; Hiller, Karl-Anton ; Schmalz, Gottfried ; Ruhl, Stefan



Abstract

For quantification of bacterial adherence to biomaterial surfaces or to other surfaces prone to biofouling, there is a need for methods that allow a comparative anal. of small material specimens. A new method for quantification of surface-attached biotinylated bacteria was established by in situ detection with fluorescence-labeled avidin-D. This method was evaluated utilizing a silicon wafer ...

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