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Methods for identification of cGKI substrates

Salb, Katharina and Schlossmann, Jens (2013) Methods for identification of cGKI substrates. In: UNSPECIFIED Methods in Molecular Biology, 1020. Methods in Molecular Biology, pp. 147-162.

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The cGMP-dependent protein kinases (cGK), which belong to the family of serine/threonine kinases, exhibit their diverse functions in cells through interaction with a variety of substrate proteins. Several substrates were identified and the interactions studied using different methods inter alia co-immunoprecipitation (Co-IP) and cGMP-agarose affinity purification. In the following chapter, we ...


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Item type:Book section
Additional Information (public):vol. title: Guanylate Cyclase and Cyclic GMP : Methods and Protocols
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Pharmacology and Toxicology (Prof. Schlossmann, formerly Prof. Seifert)
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Keywords:cGMP - cGMP-dependent protein kinases - cGKI substrate proteins - Affinity purification - Co-immunoprecipitation - cGMP-agarose
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:28263
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