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How Subvisible Particles Become Invisible - Relevance of the Refractive Index for Protein Particle Analysis

Zölls, Sarah ; Gregoritza, Manuel ; Tantipolphan, Ruedeeporn ; Wiggenhorn, Michael ; Winter, Gerhard ; Friess, Wolfgang ; Hawe, Andrea



Abstract

The aim of the present study was to quantitatively assess the relevance of transparency and refractive index (RI) on protein particle analysis by the light-based techniques light obscuration (LO) and Micro-Flow Imaging (MFI). A novel method for determining the RI of protein particles was developed and provided an RI of 1.41 for protein particles from two different proteins. An increased RI of the ...

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