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Activation of a chimeric Rpb5/RpoH subunit using library selection

Sommer, Bettina, Waege, Ingrid, Pöllmann, David, Seitz, Tobias, Thomm, Michael, Sterner, Reinhard and Hausner, Winfried (2014) Activation of a chimeric Rpb5/RpoH subunit using library selection. Plos One 9.

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Rpb5 is a general subunit of all eukaryotic RNA polymerases which consists of a N-terminal and a C-terminal domain. The corresponding archaeal subunit RpoH contains only the conserved C-terminal domain without any N-terminal extensions. A chimeric construct, termed rp5H, which encodes the N-terminal yeast domain and the C-terminal domain from Pyrococcus furiosus is unable to complement the lethal ...


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Item type:Article
Date:29 January 2014
Institutions:Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Michael Thomm
Projects:Open Access Publizieren (DFG)
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Dewey Decimal Classification:500 Science > 570 Life sciences
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:29534
Owner only: item control page


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