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Activation of a chimeric Rpb5/RpoH subunit using library selection

URN to cite this document:
Sommer, Bettina ; Waege, Ingrid ; Pöllmann, David ; Seitz, Tobias ; Thomm, Michael ; Sterner, Reinhard ; Hausner, Winfried
License: Creative Commons Attribution 3.0
PDF - Published Version
Date of publication of this fulltext: 12 Feb 2014 12:42


Rpb5 is a general subunit of all eukaryotic RNA polymerases which consists of a N-terminal and a C-terminal domain. The corresponding archaeal subunit RpoH contains only the conserved C-terminal domain without any N-terminal extensions. A chimeric construct, termed rp5H, which encodes the N-terminal yeast domain and the C-terminal domain from Pyrococcus furiosus is unable to complement the lethal ...


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