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Two-Photon Microscopy Reveals Stationary Podocytes in Living Zebrafish Larvae

Endlich, Nicole ; Simon, Ole ; Goepferich, Achim ; Wegner, Henny ; Moeller, Marcus J. ; Rumpel, Elisabeth ; Kotb, Ahmed ; Endlich, Karlhans



Abstract

Podocytes are an essential component of the glomerular filtration barrier and cover the outer aspect of glomerular capillaries. They form a complex actin-based cytoskeleton in vivo and show prominent motility in vitro, but whether podocytes are stationary or mobile in vivo is debated. To address this question, the pronephros of translucent zebrafish larvae (casper) expressing enhanced green ...

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