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Metabolite extraction from adherently growing mammalian cells for metabolomics studies: optimization of harvesting and extraction protocols

Dettmer, Katja, Nürnberger, Nadine, Kaspar, Hannelore, Gruber, Michael, Almstetter, Martin F. and Oefner, Peter J. (2011) Metabolite extraction from adherently growing mammalian cells for metabolomics studies: optimization of harvesting and extraction protocols. Analytical and bioanalytical chemistry 399 (3), pp. 1127-1139.

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Abstract

Trypsin/ethylenediaminetetraacetic acid (EDTA) treatment and cell scraping in a buffer solution were compared for harvesting adherently growing mammalian SW480 cells for metabolomics studies. In addition, direct scraping with a solvent was tested. Trypsinated and scraped cell pellets were extracted using seven different extraction protocols including pure methanol, methanol/water, pure acetone, ...

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Item type:Article
Date:January 2011
Institutions:Medicine > Lehrstuhl für Anästhesiologie
Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Identification Number:
ValueType
21125262PubMed ID
10.1007/s00216-010-4425-xDOI
Classification:
NotationType
2-Propanol/chemistryMESH
Acetone/chemistryMESH
Amino Acids/metabolismMESH
Cell Adhesion/drug effectsMESH
Cell Proliferation/drug effectsMESH
Chloroform/chemistryMESH
Edetic Acid/pharmacologyMESH
Energy Metabolism/drug effectsMESH
Gas Chromatography-Mass SpectrometryMESH
HumansMESH
Methanol/chemistryMESH
Solvents/chemistryMESH
Trypsin/pharmacologyMESH
Tumor Cells, CulturedMESH
Water/chemistryMESH
Keywords:Metabolomics GC-MS Adherent cells Detachment Extraction Amino acids
Dewey Decimal Classification:500 Science > 500 Natural sciences & mathematics
500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:30623
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