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Comparison of derivatization and chromatographic methods for GC-MS analysis of amino acid enantiomers in physiological samples

Waldhier, Magdalena Ch. ; Dettmer, Katja ; Gruber, Michael ; Oefner, Peter J.


GC-MS analysis of fluorinated and non-fluorinated chloroformate and anhydride derivatives of amino acid (AA) enantiomers on two different chiral columns was compared for the direct quantification of free L- and D-AAs in human serum and urine in a single analytical run. Best sensitivity was achieved with pentafluoropropionic anhydride/heptafluorobutanol derivatives separated on a Chirasil-L-Val ...


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