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Urinary amino acid analysis: a comparison of iTRAQ-LC-MS/MS, GC-MS, and amino acid analyzer

Kaspar, Hannelore ; Dettmer, Katja ; Chan, Queenie ; Daniels, Scott ; Nimkar, Subodh ; Daviglus, Martha L. ; Stamler, Jeremiah ; Elliott, Paul ; Oefner, Peter J.


Urinary amino acid analysis is typically done by cation-exchange chromatography followed by post-column derivatization with ninhydrin and UV detection. This method lacks throughput and specificity. Two recently introduced stable isotope ratio mass spectrometric methods promise to overcome those shortcomings. Using two blinded sets of urine replicates and a certified amino acid standard, we ...


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