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Urinary amino acid analysis: a comparison of iTRAQ-LC-MS/MS, GC-MS, and amino acid analyzer

Kaspar, Hannelore, Dettmer, Katja, Chan, Queenie, Daniels, Scott, Nimkar, Subodh, Daviglus, Martha L., Stamler, Jeremiah, Elliott, Paul and Oefner, Peter J. (2009) Urinary amino acid analysis: a comparison of iTRAQ-LC-MS/MS, GC-MS, and amino acid analyzer. Journal of chromatography B 877 (20-21), pp. 1838-1846.

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Abstract

Urinary amino acid analysis is typically done by cation-exchange chromatography followed by post-column derivatization with ninhydrin and UV detection. This method lacks throughput and specificity. Two recently introduced stable isotope ratio mass spectrometric methods promise to overcome those shortcomings. Using two blinded sets of urine replicates and a certified amino acid standard, we ...

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Item type:Article
Date:July 2009
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Identification Number:
ValueType
19481989PubMed ID
10.1016/j.jchromb.2009.05.019DOI
Classification:
NotationType
AdultMESH
Amino Acids/urineMESH
Chromatography, Liquid/methodsMESH
FemaleMESH
Gas Chromatography-Mass Spectrometry/methodsMESH
HumansMESH
MaleMESH
Middle AgedMESH
Reproducibility of ResultsMESH
Tandem Mass Spectrometry/methodsMESH
Dewey Decimal Classification:500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Partially
Item ID:30651
Owner only: item control page
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