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Modelling cross-hybridization on phylogenetic DNA microarrays increases the detection power of closely related species

Engelmann, Julia C. ; Rahmann, Sven ; Wolf, Matthias ; Schultz, Jörg ; Fritzilas, Epameinondas ; Kneitz, Susanne ; Dandekar, Thomas ; Müller, Tobias



Abstract

DNA microarrays are a popular technique for the detection of microorganisms. Several approaches using specific oligomers targeting one or a few marker genes for each species have been proposed. Data analysis is usually limited to call a species present when its oligomer exceeds a certain intensity threshold. While this strategy works reasonably well for distantly related species, it does not work ...

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