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Imaging of cellular oxygen via two-photon excitation of fluorescent sensor nanoparticles

Wang, Xu-dong ; Achatz, Daniela E. ; Hupf, Christina ; Sperber, Michaela ; Wegener, Joachim ; Bange, Sebastian ; Lupton, John M. ; Wolfbeis, Otto S.


Polystyrene nanoparticles (PSNPs) with an average size of 85 nm and loaded with an oxygen-quenchable luminescent ruthenium complex were used to sense and image oxygen inside cells following 2-photon excitation (2-PE). The ruthenium probe possesses a large two-photon absorption cross-section, and 2-PE is achieved by irradiation in the near infrared with commercially available fs-pulsed laser ...


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