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Imaging of cellular oxygen via two-photon excitation of fluorescent sensor nanoparticles

Wang, Xu-dong, Achatz, Daniela E., Hupf, Christina, Sperber, Michaela, Wegener, Joachim, Bange, Sebastian, Lupton, John M. and Wolfbeis, Otto S. (2013) Imaging of cellular oxygen via two-photon excitation of fluorescent sensor nanoparticles. Sensors and Actuators B Chemical 188, pp. 257-262.

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Other URL: http://www.sciencedirect.com/science/article/pii/S0925400513007788, http://dx.doi.org/10.1016/j.snb.2013.06.087


Abstract

Polystyrene nanoparticles (PSNPs) with an average size of 85 nm and loaded with an oxygen-quenchable luminescent ruthenium complex were used to sense and image oxygen inside cells following 2-photon excitation (2-PE). The ruthenium probe possesses a large two-photon absorption cross-section, and 2-PE is achieved by irradiation in the near infrared with commercially available fs-pulsed laser ...

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Item type:Article
Date:5 July 2013
Institutions:Physics > Institute of Experimental and Applied Physics > Chair Professor Lupton > Group John Lupton
Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:
ValueType
10.1016/j.snb.2013.06.087DOI
Keywords:Oxygen sensor; Fluorescence; Two-photon excitation; Quenching; Nanosensor; Intracellular sensing; COMPLEXES; MICROSCOPY; INDICATORS; CANCER; CELLS; LIGHT
Dewey Decimal Classification:500 Science > 530 Physics
500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:30805
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