Pyrococcus furiosus flagella: biochemical and transcriptional analyses identify the newly detected flaB0 gene to encode the major flagellin

URN to cite this document:: urn:nbn:de:bvb:355-epub-310040
DOI to cite this document:: 10.5283/epub.31004

Bellack, Annett ; Näther-Schindler, Daniela J. ; Schopf, Simone ; Rachel, Reinhard ; Wirth, Reinhard

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Date of publication of this fulltext: 26 Nov 2014 08:50

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Abstract

We have described previously that the flagella of the Euryarchaeon Pyrococcus furiosus are multifunctional cell appendages used for swimming, adhesion to surfaces and formation of cell-cell connections. Here, we characterize these organelles with respect to their biochemistry and transcription. Flagella were purified by shearing from cells followed by CsCl-gradient centrifugation and were found ...