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Enzyme Molecules in Solitary Confinement

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Liebherr, Raphaela ; Gorris, H. H.
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Date of publication of this fulltext: 04 Feb 2015 14:59


Large arrays of homogeneous microwells each defining a femtoliter volume are a versatile platform for monitoring the substrate turnover of many individual enzyme molecules in parallel. The high degree of parallelization enables the analysis of a statistically representative enzyme population. Enclosing individual enzyme molecules in microwells does not require any surface immobilization step and ...


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