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Protein compatibility of selected cross-linking reactions for hydrogels

Hammer, Nadine ; Brandl, Ferdinand ; Kirchhof, Susanne ; Messmann, Viktoria ; Goepferich, Achim



Abstract

The compatibility of selected cross-linking reactions with lysozyme is investigated. Michael-type additions of nucleophilic amino acids to maleimide, vinyl sulfone and acrylamide groups are detected by gel electrophoresis. The degree of modification depends on the polymer and the pH. Complete modification with more than five PEG chains is observed after incubation with mPEG5k-vinyl sulfone at pH ...

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