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Urinary metabolite quantification employing 2D NMR spectroscopy

Gronwald, Wolfram ; Klein, M. S. ; Kaspar, Hannelore ; Fagerer, S. R. ; Nürnberger, N. ; Dettmer, Katja ; Bertsch, T. ; Oefner, Peter J.


Two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy is a fairly novel method for the quantification of metabolites in biological fluids and tissue extracts. We show in this contribution that, compared to 1D 1H spectra, superior quantification of human urinary metabolites is obtained from 2D 1H−13C heteronuclear single-quantum correlation (HSQC) spectra measured at natural ...


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