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Automated GC-MS analysis of free amino acids in biological fluids

Kaspar, Hannelore, Dettmer, Katja, Gronwald, Wolfram and Oefner, Peter J. (2008) Automated GC-MS analysis of free amino acids in biological fluids. J. Chromatogr. B 870 (2), pp. 222-232.

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Other URL: http://www.sciencedirect.com/science/article/pii/S1570023208004376


Abstract

A gas chromatography–mass spectrometry (GC–MS) method was developed for the quantitative analysis of free amino acids as their propyl chloroformate derivatives in biological fluids. Derivatization with propyl chloroformate is carried out directly in the biological samples without prior protein precipitation or solid-phase extraction of the amino acids, thereby allowing automation of the entire ...

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Item type:Article
Date:July 2008
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Identification Number:
ValueType
10.1016/j.jchromb.2008.06.018DOI
Keywords:Metabolomics; Amino acids; Gas chromatography–mass spectrometry; Urine; Plasma; Alkyl chloroformate
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:33609
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