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Automated GC-MS analysis of free amino acids in biological fluids

Kaspar, Hannelore ; Dettmer, Katja ; Gronwald, Wolfram ; Oefner, Peter J.


A gas chromatography–mass spectrometry (GC–MS) method was developed for the quantitative analysis of free amino acids as their propyl chloroformate derivatives in biological fluids. Derivatization with propyl chloroformate is carried out directly in the biological samples without prior protein precipitation or solid-phase extraction of the amino acids, thereby allowing automation of the entire ...


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