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Kinetics of Hyal1 and PH-20 hyaluronidases: comparison of minimal substrates and analysis of the transglycosylation reaction.

Hofinger, Edith S. A. ; Bernhardt, Günther ; Buschauer, Armin


The availability of recombinant expression systems for the production of purified human hyaluronidases PH-20 and Hyal-1 facilitated the first detailed analysis of the enzymatic reaction products. The human recombinant enzymes, both expressed by Drosophila Schneider-2 (DS-2) cells, were compared to bovine testicular hyaluronidase (BTH), a commercially available hyaluronidase preparation, which has ...


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