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Fluorescence- and luminescence-based methods for the determination of affinity and activity of neuropeptide Y(2) receptor ligands.

Ziemek, Ralf ; Brennauer, Albert ; Schneider, Erich ; Cabrele, Chiara ; Beck-Sickinger, Annette G. ; Bernhardt, Günther ; Buschauer, Armin


With respect to the discovery and characterization of neuropeptide Y(2) receptor ligands as pharmacological tools or potential drugs, fluorescence- and luminescence-based assays were developed to determine both the affinity and the activity of receptor agonists and antagonists. A flow cytometric binding assay is described for the hY(2) receptor stably expressed in CHO cells using cy5-labeled ...


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