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Quantitation of hyaluronidases by the Morgan-Elson reaction: comparison of the enzyme activities in the plasma of tumor patients and healthy volunteers

Muckenschnabel, Ingo ; Bernhardt, Günther ; Spruß, Thilo ; Dietl, Barbara ; Buschauer, Armin



Abstract

The Morgan-Elson reaction, a method for the detn. of hyaluronidase activity, was optimized for the quantitation of the enzyme in biol. material. Based on HPLC and spectrometric (UV-Vis, LC-MS) studies, the structure of the red-colored product (mesomeric forms of N3-protonated 3-acetylimino-2-(4-dimethylaminophenyl)methylidene-5-(1,2-dihydroxyethyl)furane) formed by condensation of chromogen III ...

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