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Structure of recombinant human parathyroid hormone in solution using multidimensional NMR spectroscopy

Gronwald, Wolfram ; Schomburg, D. ; Harder, M. P. ; Mayer, H. ; Paulsen, J. ; Wingender, E. ; Wray, V.



Abstract

The solution structure of human parathyroid hormone, in the form of recombinant prolyl-hPTH(1-84), has been investigated by multidimensional NMR spectroscopy under conditions (aqueous trifluoroethanol) which favour the structured-state of the protein. Spin systems were identified from 3D 1H DQF (double-quantum filtered)-COSY and TOCSY spectra and sequence-specific assignments were from 2D 1H ...

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