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Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast

Sinha, H. ; David, L. ; Pascon, R. C. ; Clauder-Münster, S. ; Krishnakumar, S. ; Nguyen, M. ; Shi, G. ; Dean, J. ; Davis, R. W. ; Oefner, Peter J. ; McCusker, J. H. ; Steinmetz, L. M.


Several quantitative trait loci (QTL) mapping strategies can successfully identify major-effect loci, but often have poor success detecting loci with minor effects, potentially due to the confounding effects of major loci, epistasis, and limited sample sizes. To overcome such difficulties, we used a targeted backcross mapping strategy that genetically eliminated the effect of a previously ...


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