Assessment of Different Expression Strategies for the Production of a Recombinant Lipoprotein Vaccine in Plants

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urn:nbn:de:bvb:355-epub-345373 Copy

Hennig, A. ; Reinders, Yvonne ; Giritch, A. ; Reinders, Jörg ; Warzecha, H.

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Date of publication of this fulltext: 07 Sep 2016 08:03

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Details

Item type:	Article
Journal or Publication Title:	Open Biotechnology Journal
Publisher:	Bentham Open
Volume:	2
Page Range:	pp. 51-55
Date:	2008
Institutions:	Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Identification Number:	Value Type 10.2174/1874070700802010051 DOI
Keywords:	Plant vaccine, lipoprotein, protein palmitoylation, outer surface protein A, chloroplast transformation
Dewey Decimal Classification:	500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Yes
Item ID:	34537

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Abstract

The ability of plants to serve as a production system for bacterial lipoprotein vaccines has been investigated. First, the effect of high-level expression of the Borrelia burgdorferi outer membrane protein A (OspA), a prototype vaccine against Lyme disease, has been examined by a proteomics approach. Analysis by 2D-PAGE of wild type tobacco plants and transplastomic plants accumulating ...

Abstract

The ability of plants to serve as a production system for bacterial lipoprotein vaccines has been investigated. First, the effect of high-level expression of the Borrelia burgdorferi outer membrane protein A (OspA), a prototype vaccine against Lyme disease, has been examined by a proteomics approach. Analysis by 2D-PAGE of wild type tobacco plants and transplastomic plants accumulating recombinant OspA showed no apparent differences in protein pattern except for OspA. However, presence of the bacterial signal sequence limits transgene accumulation. As an alternative approach OspA was produced in Nicotiana benthamiana plants by transient expression via a deconstructed tobacco mosaic virus-based system. While rapid expression of OspA could be achieved, no palmitoylation occurred with the genuine bacterial sequence. In contrast, modification of the N-terminus with an eukaryotic sequence motif resulted in palmitoylation of OspA. This study shows that plants provide multiple expression strategies and could serve as a versatile production platform for recombinant lipidated subunit vaccines.

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Metadata last modified: 01 Jun 2018 16:45

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