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Quantification of metabolites by NMR spectroscopy in the presence of protein

Wallmeier, Jens, Samol, Claudia, Ellmann, Lisa, Zacharias, Helena, Vogl, Franziska C., Garcia, Muriel, Dettmer, Katja , Oefner, Peter J. and Gronwald, Wolfram (2017) Quantification of metabolites by NMR spectroscopy in the presence of protein. Journal of Proteome Research 1535-3893.

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Other URL: http://pubs.acs.org/doi/abs/10.1021/acs.jproteome.7b00057


Abstract

The high reliability of NMR spectroscopy makes it an ideal tool for large-scale metabolomic studies. However, the complexity of biofluids and, in particular, the presence of macromolecules poses a significant challenge. Ultrafiltration and protein precipitation are established means of deproteinization and recovery of free or total metabolite content, but neither is ever complete. In addition, ...

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Item type:Article
Date:15 March 2017
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Identification Number:
ValueType
28294621PubMed ID
10.1021/acs.jproteome.7b00057DOI
Keywords:MAGNETIC-RESONANCE-SPECTROSCOPY; H-1-NMR SPECTROSCOPY; NEPHROTIC SYNDROME; BLOOD-PLASMA; SERUM; H-1; METABOLOMICS; METABONOMICS; TRYPTOPHAN; EXTRACTS; ID NMR; quantification; plasma; CPMG; correction factors
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:35433
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