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Expanding the Scope of Impedance Spectroscopy for the Analysis of Adherent Cells: Electrode Material, Electrode Design, and Data Analysis

URN to cite this document:
urn:nbn:de:bvb:355-epub-364228
DOI to cite this document:
10.5283/epub.36422
Götz, Christian
Date of publication of this fulltext: 16 Oct 2018 07:25


Abstract (English)

This thesis focused on the advancement of impedance-based cellular assays using the electric cell-substrate impedance sensing (ECIS) approach. Three linchpins of ECIS were addressed: electrode material, electrode design, and data analysis. Typically, ECIS measurements are performed by application of ac currents with low amplitude between coplanar gold-film electrodes with adherent cell monolayers ...

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Translation of the abstract (German)

Das Ziel dieser Arbeit war die Weiterentwicklung der impedimetrischer Verfahren zur Analyse lebender adhärenter Zellen in vitro mittels Electric Cell-Substrate Impedance Sensing (ECIS). Dabei standen drei zentrale Themenbereiche dieses Ansatzes im Fokus: das verwendete Elektrodenmaterial, das Elektrodendesign und die Datenanalyse. Typischerweise werden ECIS-Messungen durch Anlegen niedriger ...

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