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Expanding the Scope of Impedance Spectroscopy for the Analysis of Adherent Cells: Electrode Material, Electrode Design, and Data Analysis

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Götz, Christian
Date of publication of this fulltext: 16 Oct 2018 07:25

Abstract (English)

This thesis focused on the advancement of impedance-based cellular assays using the electric cell-substrate impedance sensing (ECIS) approach. Three linchpins of ECIS were addressed: electrode material, electrode design, and data analysis. Typically, ECIS measurements are performed by application of ac currents with low amplitude between coplanar gold-film electrodes with adherent cell monolayers ...


Translation of the abstract (German)

Das Ziel dieser Arbeit war die Weiterentwicklung der impedimetrischer Verfahren zur Analyse lebender adhärenter Zellen in vitro mittels Electric Cell-Substrate Impedance Sensing (ECIS). Dabei standen drei zentrale Themenbereiche dieses Ansatzes im Fokus: das verwendete Elektrodenmaterial, das Elektrodendesign und die Datenanalyse. Typischerweise werden ECIS-Messungen durch Anlegen niedriger ...


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