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Unbound fraction of fluconazole and linezolid in human plasma as determined by ultrafiltration: Impact of membrane type

Kratzer, Alexander ; Kees, Frieder ; Dorn, Christoph


Ultrafiltration is a rapid and convenient method to determine the free concentrations of drugs in plasma. Several ultrafiltration devices based on Eppendorf cups are commercially available, but are not validated for such use by the manufacturer. Plasma pH, temperature and relative centrifugal force as well as membrane type can influence the results. In the present work, we developed an ...


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