Felthaus, Oliver ; Prantl, Lukas ; Skaff-Schwarze, Mona ; Klein, Silvan ; Anker, Alexandra ; Ranieri, Marco ; Kuehlmann, Britta
Alternative Links zum Volltext:DOIVerlag
| Dokumentenart: | Artikel |
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| Titel eines Journals oder einer Zeitschrift: | Clinical Hemorheology and Microcirculation |
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| Verlag: | IOS PRESS |
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| Ort der Veröffentlichung: | AMSTERDAM |
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| Band: | 66 |
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| Nummer des Zeitschriftenheftes oder des Kapitels: | 1 |
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| Seitenbereich: | S. 47-55 |
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| Datum: | 2017 |
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| Institutionen: | Medizin > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie |
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| Identifikationsnummer: | | Wert | Typ |
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| 10.3233/CH-160203 | DOI |
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| Stichwörter / Keywords: | VIVO BONE-FORMATION; ASSISTED LIPOTRANSFER; FAT TRANSPLANTATION; GROWTH-FACTORS; BASIC SCIENCE; BROWN; TERM; PROLIFERATION; LIPOSUCTION; EXPRESSION; Adipose tissue derived stem cells; platelet rich plasma; tissue engineering |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Ja |
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| Dokumenten-ID: | 38466 |
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Web of Science
Zusammenfassung
BACKGROUND: Autologous fat grafts and adipose-derived stem cells (ASCs) can be used to treat soft tissue defects. However, the results are inconsistent and sometimes comprise tissue resorption and necrosis. This might be due to insufficient vascularization. Platelet-rich plasma (PRP) is a source of concentrated autologous platelets. The growth factors and cytokines released by platelets can ...
Zusammenfassung
BACKGROUND: Autologous fat grafts and adipose-derived stem cells (ASCs) can be used to treat soft tissue defects. However, the results are inconsistent and sometimes comprise tissue resorption and necrosis. This might be due to insufficient vascularization. Platelet-rich plasma (PRP) is a source of concentrated autologous platelets. The growth factors and cytokines released by platelets can facilitate angiogenesis. The simultaneous use of PRP might improve the regeneration potential of fat grafts. The optimal ratio has yet to be elucidated. A byproduct of PRP preparation is platelet-poor plasma (PPP). OBJECTIVE: In this study we investigated the influence of different concentrations of PRP on the vitality and differentiation of ASCs. METHODS: We processed whole blood with the Arthrex Angel centrifuge and isolated ASCs from the same donor. We tested the effects of different PRP and PPP concentrations on the vitality using resazurin assays and the differentiation of ASCs using oil-red staining. RESULTS: Both cell vitality and adipogenic differentiation increase to a concentration of 10% to 20% PRP. With a PRP concentration of 30% cell vitality and differentiation decrease. CONCLUSIONS: Both PRP and PPP can be used to expand ASCs without xenogeneic additives in cell culture. A PRP concentration above 20% has inhibitory effects.
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