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Caging and Photoactivation in Single-Molecule Förster Resonance Energy Transfer Experiments

Jazi, Atieh Aminian, Ploetz, Evelyn , Arizki, Muhamad, Dhandayuthapani, Balasubramaniam, Waclawska, Izabela, Krämer, Reinhard, Ziegler, Christine and Cordes, Thorben (2017) Caging and Photoactivation in Single-Molecule Förster Resonance Energy Transfer Experiments. Biochemistry 56 (14), pp. 2031-2041.

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Other URL: http://doi.org/10.1021/acs.biochem.6b00916


Abstract

Caged organic fluorophores are established tools for localization-based super-resolution imaging. Their use relies on reversible deactivation of standard organic fluorophores by chemical reduction or commercially available caged dyes with ON switching of the fluorescent signal by ultraviolet (UV) light. Here, we establish caging of cyanine fluorophores and caged rhodamine dyes, i.e., chemical ...

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Item type:Article
Date:2017
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie
Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Christine Ziegler
Identification Number:
ValueType
10.1021/acs.biochem.6b00916DOI
Keywords:INDUCED FLUORESCENCE ENHANCEMENT; PHOTOINDUCED ELECTRON-TRANSFER; ALTERNATING-LASER EXCITATION; CONFORMATIONAL DYNAMICS; CORYNEBACTERIUM-GLUTAMICUM; STRUCTURAL BIOLOGY; BETAINE TRANSPORTER; OPEN COMPLEX; FRET; DYES;
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:39090
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