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Caging and Photoactivation in Single-Molecule Förster Resonance Energy Transfer Experiments

Jazi, Atieh Aminian ; Ploetz, Evelyn ; Arizki, Muhamad ; Dhandayuthapani, Balasubramaniam ; Waclawska, Izabela ; Krämer, Reinhard ; Ziegler, Christine ; Cordes, Thorben



Abstract

Caged organic fluorophores are established tools for localization-based super-resolution imaging. Their use relies on reversible deactivation of standard organic fluorophores by chemical reduction or commercially available caged dyes with ON switching of the fluorescent signal by ultraviolet (UV) light. Here, we establish caging of cyanine fluorophores and caged rhodamine dyes, i.e., chemical ...

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