Abstract
A simple, accurate and selective column-switching high-performance liquid chromatography (HPLC) method was developed and validated for simultaneous quantification of six beta-blockers (metoprolol, timolol, bisoprolol, propranolol, carvedilol and nebivolol), three of their metabolites (-hydroxy metoprolol, N-desisopropyl propranolol and 4-hydroxy carvedilol 4-HCAR), three antipsychotics ...
Abstract
A simple, accurate and selective column-switching high-performance liquid chromatography (HPLC) method was developed and validated for simultaneous quantification of six beta-blockers (metoprolol, timolol, bisoprolol, propranolol, carvedilol and nebivolol), three of their metabolites (-hydroxy metoprolol, N-desisopropyl propranolol and 4-hydroxy carvedilol 4-HCAR), three antipsychotics (olanzapine, clozapine and quetiapine) and three of their metabolites (N-desmethyl olanzapine, N-desmethyl clozapine and N-desalkyl quetiapine) in human serum. After pretreatment on a Merck LiChrospher RP-4 ADS column (25m), drugs were separated on a Phenomenex Gemini Phenyl Hexyl 110 A column (250x4.6mm, 5m) using a gradient mixture of acetonitrile and potassium dihydrogen phosphate buffer pH3.1 (containing 10% methanol) as a mobile phase at a flow rate of 1mL/min. The total analysis time was 40min. For detection of the analytes, four different UV wavelengths were used: 215, 226, 242 and 299nm. The method was validated according to the guidelines of the Society of Toxicology and Forensic Chemistry in terms of selectivity, linearity, accuracy, precision and stability and successfully applied for the analysis of the 15 described analytes in human serum.
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