Dokumentenart: | Artikel | ||||
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Titel eines Journals oder einer Zeitschrift: | Respiratory Physiology & Neurobiology | ||||
Verlag: | ELSEVIER SCIENCE BV | ||||
Ort der Veröffentlichung: | AMSTERDAM | ||||
Band: | 244 | ||||
Seitenbereich: | S. 17-25 | ||||
Datum: | 2017 | ||||
Institutionen: | Medizin > Lehrstuhl für Anästhesiologie Biologie und Vorklinische Medizin > Institut für Physiologie Biologie und Vorklinische Medizin > Institut für Physiologie > Prof. Dr. Richard Warth | ||||
Identifikationsnummer: |
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Stichwörter / Keywords: | CEREBELLAR GRANULE NEURONS; DOMAIN K+ CHANNEL; CAROTID-BODY; ARTERIAL CHEMORECEPTORS; INHALATION ANESTHETICS; VENTILATORY RESPONSES; PH SENSITIVITY; GLOMUS CELLS; OXYGEN; CO2; TASK potassium channels; Chemoreception; Whole body plethysmograph; Hyperoxia | ||||
Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin | ||||
Status: | Veröffentlicht | ||||
Begutachtet: | Ja, diese Version wurde begutachtet | ||||
An der Universität Regensburg entstanden: | Ja | ||||
Dokumenten-ID: | 39758 |
Zusammenfassung
Despite intensive research, the exact function of TASK potassium channels in central and peripheral chemoreception is still under debate. In this study, we investigated the respiration of unrestrained TASK-3 (TASK-3(-/-)) and TASK-1/TASK-3 double knockout (TASK-1/3(-/-)) adult male mice in vivo using a plethysmographic device. Ventilation parameters of TASK-3/mice were normal under control ...
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Zusammenfassung
Despite intensive research, the exact function of TASK potassium channels in central and peripheral chemoreception is still under debate. In this study, we investigated the respiration of unrestrained TASK-3 (TASK-3(-/-)) and TASK-1/TASK-3 double knockout (TASK-1/3(-/-)) adult male mice in vivo using a plethysmographic device. Ventilation parameters of TASK-3/mice were normal under control condition (21% 02) and upon hypoxia and hypercapnia they displayed the physiological increase of ventilation. TASK-1/3(-/-) mice showed increased ventilation under control conditions. This increase of ventilation was caused by increased tidal volumes (V-T), a phenomenon similarly observed in TASK-1(-/-) mice. Under acute hypoxia, TASK-1/3(-/-) mice displayed the physiological increase of the minute volume. Interestingly, this increase was not related to an increase of the respiratory frequency (f(R)), as observed in wild-type mice, but was caused by a strong increase of V-T. This particular respiratory phenotype is reminiscent of the respiratory phenotype of carotid body-denervated rodents in the compensated state. Acute hypercapnia (5% CO2) stimulated ventilation in TASK-1/3 i and wild-type mice to a similar extent; however, at higher CO2 concentrations ( > 5% CO2) the stimulation of ventilation was more pronounced in TASK-1/3(-/-) mice. At hyperoxia (100% O-2), TASK-1(-/-) , TASK-3(-/-) and wild-type mice showed the physiological small decrease of ventilation. In sharp contrast, TASK-1/3(-/-) mice exhibited an abnormal increase of ventilation under hyperoxia. In summary, these measurements showed a grossly normal respiration of TASK-3(-/-) mice and a respiratory phenotype of TASK-1/3(-/-) mice that was characterized by a markedly enhanced tidal volume, similar to the one observed in TASK-1(-/-) mice. The abnormal hyperoxia response, exclusively found in TASK-1/3(-/-) double mutant mice, indicates that both TASK-1(-/-) and TASK-3 are essential for the hyperoxia-induced hypoventilation. The peculiar respiratory phenotype of TASK-1/3(-/-) knockout mice is reminiscent of the respiration of animals with long-term carotid body dysfunction. Taken together, TASK-1 and TASK-3 appear to serve specific and distinct roles in the complex processes underlying chemoreception and respiratory control.
Metadaten zuletzt geändert: 25 Nov 2020 15:47