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Comparative purification and characterization of hepatitis B virus-like particles produced by recombinant vaccinia viruses in human hepatoma cells and human primary hepatocytes

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Shih, Chiaho ; Reuschel, Edith ; Jilg, Wolfgang ; Seelbach-Goebel, Birgit ; Deml, Ludwig
Date of publication of this fulltext: 03 May 2019 10:20


This study describes the comparative expression and purification of hepatitis B surface antigen (HBsAg) particles produced upon infection of human primary hepatocytes and human hepatoma cell lines (HuH-7 and HepG2) with recombinant vaccinia viruses. The highest levels of HBsAg expression were found in HuH-7 hepatoma cells following infection with recombinant vaccinia viruses, which contain the S ...


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