Comparative purification and characterization of hepatitis B virus-like particles produced by recombinant vaccinia viruses in human hepatoma cells and human primary hepatocytes

URN to cite this document:: urn:nbn:de:bvb:355-epub-401723
DOI to cite this document:: 10.5283/epub.40172

Shih, Chiaho ; Reuschel, Edith

; Jilg, Wolfgang ; Seelbach-Goebel, Birgit ; Deml, Ludwig

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Date of publication of this fulltext: 03 May 2019 10:20

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Abstract

This study describes the comparative expression and purification of hepatitis B surface antigen (HBsAg) particles produced upon infection of human primary hepatocytes and human hepatoma cell lines (HuH-7 and HepG2) with recombinant vaccinia viruses. The highest levels of HBsAg expression were found in HuH-7 hepatoma cells following infection with recombinant vaccinia viruses, which contain the S ...