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Comparative purification and characterization of hepatitis B virus-like particles produced by recombinant vaccinia viruses in human hepatoma cells and human primary hepatocytes

Shih, Chiaho, Reuschel, Edith , Jilg, Wolfgang, Seelbach-Goebel, Birgit and Deml, Ludwig (2019) Comparative purification and characterization of hepatitis B virus-like particles produced by recombinant vaccinia viruses in human hepatoma cells and human primary hepatocytes. PLOS ONE 14 (2), e0212800.

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Date of publication of this fulltext: 03 May 2019 10:20

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Other URL: http://doi.org/10.1371/journal.pone.0212800


Abstract

This study describes the comparative expression and purification of hepatitis B surface antigen (HBsAg) particles produced upon infection of human primary hepatocytes and human hepatoma cell lines (HuH-7 and HepG2) with recombinant vaccinia viruses. The highest levels of HBsAg expression were found in HuH-7 hepatoma cells following infection with recombinant vaccinia viruses, which contain the S ...

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Item type:Article
Date:22 February 2019
Institutions:Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Geburtshilfe)
Projects:Open Access Publizieren (DFG)
Identification Number:
ValueType
10.1371/journal.pone.0212800DOI
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:40172
Owner only: item control page

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