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Photocycle Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor QuasAr1

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Penzkofer, Alfons ; Silapetere, Arita ; Hegemann, Peter
License: Creative Commons Attribution 4.0
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Date of publication of this fulltext: 09 Jan 2020 13:33


The retinal photocycle dynamics of the fluorescent voltage sensor QuasAr1 (Archaerhodopsin 3 P60S-T80S-D95H-D106H-F161V mutant from Halorubrum sodomense) in pH 8 Tris buffer was studied. The samples were photoexcited to the first absorption band of the protonated retinal Schiff base (PRSB) Ret_580 (absorption maximum at lambda(max) approximate to 580 nm), and the retinal Schiff base ...


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