Photocycle Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor QuasAr1

URN to cite this document:: urn:nbn:de:bvb:355-epub-413517
DOI to cite this document:: 10.5283/epub.41351

Penzkofer, Alfons ; Silapetere, Arita ; Hegemann, Peter

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Date of publication of this fulltext: 09 Jan 2020 13:33

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Abstract

The retinal photocycle dynamics of the fluorescent voltage sensor QuasAr1 (Archaerhodopsin 3 P60S-T80S-D95H-D106H-F161V mutant from Halorubrum sodomense) in pH 8 Tris buffer was studied. The samples were photoexcited to the first absorption band of the protonated retinal Schiff base (PRSB) Ret_580 (absorption maximum at λmax ≈ 580 nm), and the retinal Schiff base photoisomerization and ...