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Development of a dual ECIS-SPR sensor platform for cell-based assays: Label-free analysis of g-protein coupled receptor signal transduction

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Parić, Zlatko
Date of publication of this fulltext: 09 Feb 2021 08:12

Abstract (English)

Label-free cell-based assays are an important analytical tool in drug research today. Optical approaches based on evanescent fields (e.g. Surface Plasmon Resonance, SPR; Dynamic Mass Redistribution, DMR) and altering current based impedance analysis (Electric Cell substrate Impedance Sensing, ECIS) are the most widespread techniques. The aim of this thesis was to develop and establish a dual ...


Translation of the abstract (German)

Markierungsfreie zellbasierte Assays sind heutzutage ein wichtiges analytisches Instrument in der Arzneimittelforschung. Optische Methoden die auf evaneszenten Feldern basieren (z. B. Oberflächenplasmonresonanz, SPR, dynamische Massenumverteilung, DMR) und Wechseltromwiederstand basierte Impedanzmessung (Electric Cell-Substrate Impedance Sensing, ECIS) sind die am weitesten verbreiteten ...


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