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Rapid and Precise Semi-Automatic Axon Quantification in Human Peripheral Nerves

Engelmann, S. ; Ruewe, M. ; Geis, S. ; Taeger, C. D. ; Kehrer, M. ; Tamm, E. R. ; Bleys, R. L. A. W ; Zeman, F. ; Prantl, L. ; Kehrer, A.
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Date of publication of this fulltext: 26 Feb 2020 14:24


Abstract

We developed a time-efficient semi-automated axon quantification method using freeware in human cranial nerve sections stained with paraphenylenediamine (PPD). It was used to analyze a total of 1238 facial and masseteric nerve biopsies. The technique was validated by comparing manual and semi-automated quantification of 129 (10.4%) randomly selected biopsies. The software-based method ...

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